Analysis Note

ControlL6 cell lysate

Application

Immunocytochemistry: A previous lot of this antibody was used in immunocytochemistry at a 1:500 dilution.

Immunoprecipitation: A previous lot of this antibody was used in IP; 3 µL is a recommended as starting point for immunoprecipitation.
Optimal working dilutions must be determined by end user.

Research CategorySignaling

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Anti-SG2NA Antibody is an antibody against SG2NA for use in WB, IC & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SG2NA, also known as Striatin 3, was identified as a human cancer auto-antigen and has not been implicated in vesicular trafficking and signal tranduction. It is highly related to striatin and zinedin (striatin 4). It is a cytoplasmic scaffolding protein that binds to Calmodulin and PP2A A and C subunits as well as the human homolog of the yeast protein, Mob1, which appears to modulate PP2A activity.

Immunogen

Synthetic peptide from SG2NA.

Linkage

Replaces: MAB5464

Physical form

Mouse monoclonal IgG1 ascites containing 40% glycerol and 0.02% sodium azide.

Quality

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected SG2NA on 10 µg of L6 lysates.

Specificity

Reacts with SG2NA. No cross reactivity with striatin.

Storage and Stability

Stable at -20°C in undiluted aliquots for up to 1 year from date of receipt. For long-term storage, may be stored at -80°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

The antibody recognizes the ~93 kDa SG2NA protein in Jurkat cell lysate. In some cell types a band of ~70 kDa is also recognized, which may be an alternatively spliced form of SG2NA or another unknown protein.